Intriguing Facts Related To Custom Peptide Synthesis & Challenges Involved

 

New reagents, coupling agents, and methods used in solid-phase peptide synthesis in India are overcoming the existing challenges. Let’s take a look. 

For research including the evaluation of a protein's antigenicity, prospective vaccine candidates, drug design, protein modelling, peptide-peptide interactions, peptide-drug interactions, etc., custom-synthesised peptides are in the application. Here are a few parameters you should be aware of before beginning if you are new to the topic of peptide synthesis in India.

Parameters you need to know before starting peptide synthesis

• Peptide length: Shorter peptides with a length of 2–50 amino acids (AAs) are simpler to make. The complexity of peptide synthesis rises with a length exceeding 50 AAs, despite the fact that peptides up to 108 AAs have also been created. Peptides longer than 50 AA have been successfully synthesised using solid phase synthesis, however, longer peptides still present a challenge. Longer peptides are capable of being synthesised.
• Peptide quantity: The peptides that must be produced in order: It is simpler to synthesise peptides when they are made of natural or L- Amino Acids. Some of the AAs are quite problematic; for instance, if proline is in the second position from the C-terminal, it interferes with peptide synthesis sterically. Similar to this, up until now, the synthesis of hexapeptides including all glycines has proven to be incredibly challenging. According to a reputed peptide synthesis company in India, Unnatural AAs or any alteration at the peptide's C-terminus can cause the cost of peptide synthesis to be abnormally high. A competent peptide chemist with average chemistry skills is also necessary.
• Peptide purity: Another factor that significantly affects peptide price is peptide purity. Typically, desalination using the gel filtration process yields a purity level of >70%. Through this procedure, salts and scavengers from produced peptides are removed. One can get higher purity by using the HPLC method of purification. The first HPLC run in this purification procedure results in a purity level >90%. However, more HPLC purification cycles are necessary to achieve higher purities, such as >97% or >98% purity.

Conclusion

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