What Are The Types Of ELISA? Some Basic Principles & Its Benefits

Types Of ELISA & Its benefits


Enzyme-linked immunosorbent assay is a method to determine the presence of antigens in biological samples. Phenomenal ELISA kit providers in India state its type.


Just like any other kind of immunoassay, ELISA depends on antibodies for detecting a target antigen. Elisa kits suppliers in India produce ELISA kits with highly specific antigen-antibodies interactions. Let’s know the basic principles with which an ELISA kit works. 


Key Principles Of ELISA


In an ELISA assay, antigen immobilization takes place on a solid surface. Sometimes it is done directly and some other times, with the use of a capture antibody itself immobilizing on the surface. The antigen then mixes up with a detection antibody that further conjugates with an amenable molecule for detection. The instances of such ELISA principles are fluorophore or an enzyme. 

A multi-well plate with 96- or 384-wells is ideal for performing an ELISA assay. This helps the solid surface to immobilize the antigen. Analytes immobilization further facilitates the splitting up of the antigen from other components.


Advantages Of ELISA


ELISA kits are highly specific as well as sensitive. 

It has a high throughput, however, one can easily adapt the assay to 384 well plates.

ELISA experiments are easy to conduct.

These can determine the concentration of antigen in samples.

From urine to saliva to plasma, ELISA kits can test various types of samples. 


Types Of ELISA


Generally, ELISA is found in 4 types. These are:


Direct ELISA

Indirect ELISA

Sandwich ELISA

Competitive ELISA


Regardless of the types, ELISA kits have the ability to detect antigens and antibodies at a very high rate.


Conclusion



If a reliable Elisa Kit For Food Safety & Drug Residues is what you are searching for, get in touch with Biochain. Their ELISA kits undergo a multi-step process of validation. So, head over to the CUSABIO Elisa Kit distributors and conduct meaningful life science research and experiments. 

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